Molecular Detection by blaIMPgene in Pseudomonas aeruginosa from Clinical Specimens and Relationship with Multidrug Resistance (MDR)

Document Type : Original Article

Authors

1 Department of Biology, College of Science, University of Misan, Amarah, Maysan, Iraq

2 College of Dentistry, University of Misan, Amarah, Maysan, Iraq

3 Al-Manara College for Medical Sciences, Maysan, Amarah, Iraq

Abstract

Due to its numerous resistance mechanisms, Pseudomonas aeruginosa is a significant cause of infection in hospitalized patients, leading to morbidity and mortality. As a therapy choices grows limited, the search for a new agent grows more pressing. So P. aeruginosa is an incredibly flexible Gram-negative bacterium able to flourish in a wide range of conditions, which presents major issues for doctors and nurses. One hundred specimens were gathered from clinical sources at Al-Fayhaa Hospital and Al Basrah Teaching Hospital, comprising (50) swabs from burn unit patients' exudate wounds and (50) urine specimens from patients with urinary tract infections. All of these items were cultivated in different media (89), and P. aeruginosa bacterial isolates were determined through microscopic examination and biochemical tests. A molecular diagnosis can be identified by using a traditional PCR approach to identify the specified amplification of gene product of the blaIMP gene, which was 77.55% in swabs from exudate wounds than the urine of patients with infections of the urinary tract, compared to 75% for P. aeruginosa.  Isolates of bacteria had great resistance to antibiotics, and the results revealed a high degree of resistance to gatifloxacin, ciprofloxacin (88.23% each), and gentamycin (77.94%), whereas colistin (70.14%) had low resistance from bacteria.  P. aeruginosa isolates showed multi-drug resistance (MDR). Antibiotic overuse has led to the evolution of resistant bacteria. which has resulted in inefficient treatment with antibiotics.

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