Annona squamosa (custard apple) fruit extract alleviates the oxidative stress and inflammation induced by doxorubicin in the liver of male rats

Document Type : Original Article

Authors

Biology Department, College of Science, Jazan University, Kingdom Saudi Arabia

Abstract

Background: Doxorubicin (DOX) is a commonly utilized and effective chemotherapeutic agent for treating various cancers, with side effects such as hepatotoxicity. Multiple research studies to alleviate hepatic impairment. Annona squamosa Linn is an essential medicinal plant that produces edible fruits known as custard apples. Aim: This study aimed to assess the therapeutic effect of Annona squamosa fruit extract (ASFE) against liver damage caused by doxorubicin in male rats. Materials and Methods: This study utilized thirty-two male adult albino rats. The rats were divided into four groups of eight: the control group, the DOX-treated group (3 mg/kg biweekly via intraperitoneal injection for 4 weeks), the ASFE-supplemented group (350 mg/kg body weight daily for 4 weeks), and the DOX-treated group that also received ASFE, Results: Administration of DOX to rats significantly elevated liver function and oxidative stress levels. There was evident hepatic inflammation and histological alterations. ASE effectively reinstated the impaired liver functions caused by DOX to normal levels, as seen by a notable reduction in liver enzymes (ALT and AST) and total bilirubin. ASFE substantially modified the oxidative stress caused by DOX by increasing antioxidants (SOD, CAT, and GSH) and reducing MDA levels. Similarly, the detrimental histological alterations in the liver, such as dilated and congested blood sinusoids, cellular infiltration, and fibrosis induced by DOX, seemingly resolved following treatment with ASFE. Furthermore, ASFE effectively mitigated the inflammatory effects of DOX by downregulating tumor necrosis factor-alpha (TNF-α). Conclusion: The extract from A. squamosa fruit effectively mitigated hepatic oxidative stress and inflammation induced by doxorubicin in male rats.

Keywords

Files

Share

How to cite

Statistics