Association between Single Nucleotide Polymorphism in the Promoter of CD14 Gene and Development of Urinary Tract Infection

Kasid, Mustafa; AlChalabi, Rawaa; Harith, Faisal; Aljeboury, Ghydaa H.

doi:10.21608/jmals.2023.305704

Association between Single Nucleotide Polymorphism in the Promoter of CD14 Gene and Development of Urinary Tract Infection

Document Type : Original Article

Authors

¹ Department of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Jadriya, Baghdad, Iraq

² Urology Department, Al-Yarmouk Teaching Hospital, Baghdad, Iraq

³ . Biotechnology Research Center, Al-Nahrain University, Jadriya, Baghdad, Iraq

10.21608/jmals.2023.305704

Abstract

The tetra-arm method was employed in this investigation to identify the SNP (rs2569190) in the promoter region of the CD14 gene as a risk factor for acquiring chronic urinary tract infections in a sample of Iraqis with a history of these illnesses in various forms. Using sandwich ELISA to estimate the serum level of CD14 in conjunction with TLR4 and other associated cytokines (TNF-α, TGF-β, and IFN-γ). A Gram-negative co-receptor for LPS, CD14 is expressed by innate immune cells. Through their effects on neutrophil expression levels, which have been associated with pyelonephritis, recurrent cystitis, and asymptomatic bacteriuria, its genetic variations have been connected to susceptibility to various types of UTIs The frequency of genotypes distribution and alleles of rs2569190 for two investigated groups presented The GG, GA, and AA genotype frequencies of rs2569190 were (26.1 %, 60.5 % and 13.4 %) respectively among the patients while 77.5 % for GG, 17.5% for GA and 5% for AA genotypes of healthy control.

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