Optimization of hyaluronidase enzyme conditions produced from pseudomonas aerigenosa isolated from diabetic foot ulcer patients

Document Type : Original Article

Author

Department of Medical Optics Techniques, Al-Farabi University College, Baghdad, IRAQ

Abstract

Using clinically isolated pseudomonas aerigenosa, the best conditions for making hyaluronidase were studied. The production medium pH7 with 0.5% lactose and yeast extract, a novel bacterial culture (106Cell/ml), and 37oC for 24 hours produced the maximum hyaluronidase. The specific activity of the hyaluronidase that was generated in the culture medium rose to 130 U/mg of protein as a result of these circumstances.

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